Figure 2. Differential Expression of RORγt and RORγ.

(A) Schematic representation of RORγt and RORγ cDNAs and the locations of the primers used in (B) and (C). The shaded region represents the distinct nucleotide sequences. Specific primers are 1 and 2. Common primers are 3, 4, and 5. Diagram is not to scale.

(B) Expression of RORγt and RORγ. RT-PCR products of different tissues were blotted and probed with a full-length RORγt cDNA. HPRT serves as an internal control. Numbers in parentheses indicate the primers shown in (A) used in the PCR.

(C) Expression of RORγt in thymocyte subpopulations. RT reactions of different thymocyte subpopulations were serially diluted at 1:3 and subjected to PCR with primers shown in (A). PCR products were blotted and probed as in (B). HPRT RT-PCR was performed from the same RT samples for cDNA template quantity control.