(A) RORγt does not inhibit Fas-induced killing in KMIs-8.3.5 cells. KMIs-8.3.5hCD2 control or KMIs-8.3.5RORγt cells were cocultured with L929 or L929FasL cells at a ratio of 1:3. Two days later, the percentage of hCD2 positive cells from cells cocultured with L929 (empty column) or L929FasL (filled column) were determined by FACS.
(B) RORγt does not inhibit cell death induced by dexamethasone, ceramide, and staurosporine. KMIs-8.3.5hCD2 control or KMIs-8.3.5RORγt cells were cultured in the presence of indicated stimuli and [3H]Thymidine incorporation measured. [3H]Thymidine uptake of cells cultured in medium alone is calculated as 100%. Shown are results from two individual KMIs-8.3.5RORγt clones (circles) and KMIs-8.3.5hCD2 control cells (squares).