. Author manuscript; available in PMC: 2009 Nov 12.

Published in final edited form as: Carcinogenesis. 2007 Jan 27;28(6):1269–1276. doi: 10.1093/carcin/bgm018

Table II.

Effect of CLA feeding for 10 days on proliferation and apoptosis in mammary epithelial cells from FVB wild type and FVB/erbB2 transgenic mice

Mouse strain	Mammary Structure	Variable^*	Control Diet	c9,t11-CLA Diet	t10,c12-CLA Diet
FVB	TEB	Ki67	13.6 ± 10.7^a	18.2 ± 6.4^a	45.8 ± 1.9^b
FVB	Ducts	Ki67	19.0 ± 1.7^a	5.4 ± 1.3^b	31.6 ± 1.7^c
FVB	Lobules	Ki67	15.5 ± 2.4^a	8.7 ± 2.4^a	38.4 ± 1.7^b

FVB	TEB	TUNEL	1.22 ± 0.51^a	0.83 ± 0.43^a	3.04 ± 0.54^a
FVB	Ducts	TUNEL	1.22 ± 0.28^a	0.73 ± 0.22^a	2.50 ± 0.65^a
FVB	Lobules	TUNEL	2.52 ± 0.76^a	1.03 ± 0.32^a	1.93 ± 0.56^a

FVB/erbB2	TEB	Ki67	2.74 ± 0.74^a	2.56 ± 0.46^a	38.8 ± 3.3^b
FVB/erbB2	Ducts	Ki67	7.17 ± 1.36^a	4.32 ± 0.85^a	22.4 ± 2.1^b
FVB/erbB2	Lobules	Ki67	3.06 ± 0.55^a	5.24 ± 0.87^a	26.0 ± 2.0^b

FVB/erbB2	TEB	TUNEL	0.51^a ± 0.18	0.43 ± 0.15^a	2.39 ± 0.39^b
FVB/erbB2	Ducts	TUNEL	0.35 ± 0.15^a	0.55 ± 0.21^a	3.47 ± 0.58^b
FVB/erbB2	Lobules	TUNEL	0.89 ± 0.22^a	0.08 ± 0.06^b	2.41 ± 0.42^c

Mean ± SEM.

, Percent Ki67- or TUNEL- positive nuclei. All mammary structures within each section were evaluated. Mice (n=7 per FVB group, and n=5 per FVB/erbB2 group) were fed control or CLA-supplemented diets for 10 days, starting at 70 days of age. For the control, c9,t11-CLA and t10,c12-CLA groups, respectively, the number of mammary structures counted were as follows. FVB mice (Ki67): TEBs, 5, 5, 84; ducts, 86,88, 86; lobules, 32, 20, 44. FVB/erbB2 mice (Ki67): TEBs, 60, 58, 57; ducts, 62, 60, 66; lobules, 50, 53, 57. FVB mice (TUNEL): TEBs, 10, 17, 82; ducts, 87, 87, 89; lobules, 17, 10, 37. FVB/erbB2 mice (TUNEL): TEBs, 59, 61, 60; ducts, 66, 71, 72; lobules, 52, 43, 59. Means in a row without a common letter are statistically different.