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. Author manuscript; available in PMC: 2010 Jun 3.
Published in final edited form as: Cell Cycle. 2009 Jun 3;8(11):1747–1753. doi: 10.4161/cc.8.11.8620

Figure 1.

Figure 1

H3K56 acetylation was present in mammalian cells. (A) Yeast, H1299 (Human), mouse embryonic fibroblast (Mouse) and Rat1a (Rat) cells were lysed and cell lysates were blotted with antibodies against H3K56Ac or H3. GST-H3 purified from bacteria cells were used as negative control. (B) Cells expressing FLAG-tagged wild-type H3 or K56 mutants were blotted with antibodies against H3K56Ac or the FLAG epitope. (C) H1299 cells were synchronized by double thymidine block and released into the cell cycle. Cells were harvested at indicated time points and cell extracts analyzed by western blot using indicated antibodies against H3K56Ac and H3. Cell cycle distribution at each time point was determined by fluorescence-activated cell sorting (FACS).