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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Struct Biol. 2009 Jun 21;168(2):323–331. doi: 10.1016/j.jsb.2009.06.010

Figure.4.

Figure.4

Correlative analysis of atlas maps constructed before and after specimen rotation to determine the rotational alignment parameters. Atlas maps were composed of 121 images of 1024 × 1024 pixels with 4x on-chip binning and 0.1s exposure acquired with a GATAN UltraCam CCD camera. The magnification was 480x on FEI TF30 Polara G2 electron microscope. (a) Auto-correlation image of the atlas map before specimen rotation. (b) Auto-correlation image of the atlas map after specimen rotation. (c) Atlas map before specimen rotation. (d) Atlas map after specimen rotation.