Fig. 4.

Gene expression of cartilage-to-bone-associated genes and formation of primary spongiosa are inhibited in Kif3a-deficient synchondroses. (A,F) Parasagittal serial sections of P7 control (A) and mutant so synchondrosis (F) were stained with fast green/Safranin O to reveal bone tissue. Note the marked reduction of primary bone spongiosa in mutant tissue (F) that is instead quite clear in controls (A, arrowheads). (B–E) and (G–J) Expression of indicated genes as revealed by in situ hybridization with serial sections of control (B–E) and mutant (G–J) tissue. Scale bar is 80 μm for A–J.