Fig. 5.

Intramembranous ossification is excessive near Kif3a-deficient synchondroses. Parasagittal serial sections of P7 control (A–D) and mutant so synchondroses were processed for staining with fast green/Safranin O (A,E) or in situ hybridization analysis of indicated genes. Note the presence of intramembranous bone collar adjacent to the pre-hypertrophic and hypertrophic zones in control (A–D, arrowhead) and its absence near the proliferative and resting zones (A–D, arrow) as to be expected. Note instead that intramembranous bone had formed all along the flank of the Kif3a-deficient synchondrosis (E–H). Scale bar is 75 μm for A–H.