Table 1.
Selection of short-listed antigens. The criteria for short-listed antigens included the frequency of human T cell responses to recombinant antigens expressing chlamydial antigens, biological relevance, surface exposure/antibody targets, primary sequence identity with human proteins, C. trachomatis specificity, and protection in the intra-vaginal murine challenge model
| Antigen | Hits T cells Human n=16 |
Hits T cells Murine UVEB immunized |
Hits T cells Murine AS01/UVEB challenged |
Hit Serum IgG Human n=11 |
a Reduction in Shedding day 7 Median log reduction b(# of experiment) |
a Mean Inflammatory score of UGT (# of mice) |
Expression/ Purification |
Chlamydia Specificity |
AA identity Human |
8 AA Stretch with human 100% id |
|---|---|---|---|---|---|---|---|---|---|---|
| Momp | 11 | + | + | 3 | 0.16–3 (10) | 1.1 (35) 5 (3) | Good | Chlamydia | 0 | No |
| CT460 Swib | 9 | − | − | 0 | 0.42 (1) | at 107 infection 3.1 (4) | Good | Chlamydia | Insig. | No |
| CT875 | 9 | − | − | 6 | 0 (4) | at 107 infection | Difficult Good | C.t. | 0 | No |
| pmpD-pd | 7 | + | − | 5 | 0–1.6 (4) | 2.0 (15) | (some breakdown) | C.t. | 0 | No |
| CT858 | 7 | − | − | 4 | 0.75–1 (3) | 1.3 (8) | Difficult | Chlamydia | Insig. | No |
| pmpG-pd | 6 | + | w | 0 | 0–0.25 (3) | 2.1 (4) | Good Good | C.t. | 0 | No |
| CT622 | 4 | + | + | 4 | 0–0.5 (3) | 2.0 (16) | (some breakdown Good) | Chlamydia | Insig | No |
| CT089 | 1 | − | − | n.t. | 0.33–0.6 (2) | 2.6 (4) | (some breakdown ) | Chlamydia | Insig. | No |
| CT610 | 3 | − | − | n.t. | 1 (1) | n.d. | Very good | Chlamydia | Insig. | No |
| pmpH-pd | 7 | + | − | 0 | 0–1.42 (3) | 1.5 (12) | Good N (good), C | C.t. | 0 | No |
| CT315 rpoB | 9 | − | + | 2 | 1–1.33 (3) | 1.2 (16) | (fair) | Common | 37.3 | No |
| CT322 | 9 | − | + | 1 | 0.4–1.75 (3) | 2.3 (16) | Very good | Common | 55% | Yes |
w: Weak response, only positive by proliferation.
a
Antigen screening in vaginal challenge model.
b
Number of repeated experiments. Table shows the lowest and highest reduction in bacterial shedding as compared to the AS01B sham-immunized control. UGT: Upper genital tract. Chlamydia denotes both C. trachomatis and Chlamydia pneumoniae