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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Circ Res. 2009 Aug 27;105(7):676–685. doi: 10.1161/CIRCRESAHA.109.201673

Figure 2. Phosphorylation of caveolin-1 is required for H2O2-induced endocytosis and transendothelial albumin transport.

Figure 2

(A) Confocal images showing H2O2-induced concentration-dependent increase in the uptake of Alexa 488-labeled albumin (green). The nucleus (blue) was stained with DAPI. Scale bars = 10 μm. Results are typical of 3 experiments. (B) H2O2 increased 125I-albumin endocytosis in a concentration-dependent manner. (C) H2O2 induced a concentration-dependent increase in transendothelial transport of 125I-albumin. (D,E) Effect of H2O2 on 125I-albumin endocytosis (D) and transendothelial albumin permeability (E) in cells stably expressing WT and phosphorylation-defective Y14F-Cav-1 mutant. (F,G) Effect of Src inhibition and downregulation of c-Abl kinase on H2O2-induced increase in 125I-albumin endocytosis (F) and transcytosis (G). n = 4-6 for each group. The baseline permeability values for WT and control groups were 7.8±1.2 μl·min-1·cm-2×10-2 (E) and 6.1±1.1 μl·min-1·cm-2×10-2 (G), respectively. *P <0.05 compared with control (B, C, F, and G) and WT (D, E) groups, †P <0.05 compared with respective (D,E) and control (F,G) groups.