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. 2009 Jun 24;29(25):7966–7977. doi: 10.1523/JNEUROSCI.1054-09.2009

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Copyright © 2009 Society for Neuroscience 0270-6474/09/297966-12$15.00/0

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Figure 5. — NKCC1 knockdown affects the survival rate of newborn dentate granule cells. a, Confocal images of transduced cells in control animals (shCTR/TagRFP injected) and shNKCC1/TagRFP-injected animals at 7 and 14 dpi. The percentage of double-transduced cells (yellow) in shNKCC1/TagRFP animals is decreased. shCTR- and shNKCC1-transduced cells in green, TagRFP in red. Scale bars, 25 μm. b, Quantification of the survival rate. There is a significant reduction in the fraction of double cells in the shNKCC1/TagRFP group between 7 and 14 dpi (**p < 0.01), indicating that NKCC1 knockdown affects survival of newborn neurons.