Figure 3.

Proliferation and IL-2 secretion dose response to MCC peptide of lymph node cells from 5C.C7 mice crossed with 129 wild-type or 129 vav^−/− mice are the same but differ from 5C.C7 mice on its original B10.BR background. Lymph node cells isolated from 5C.C7 mice or the crosses shown in the figures were stimulated for 48 h with B10.BR mouse splenocytes loaded with increasing amounts of MCC peptide. Data are representative of four independent experiments. (A and B) The incorporation of [³H]thymidine during the last 16–20 h of culture was measured. Experiments were performed in triplicates. (C and D) Culture supernatants of the cell cultures described in A and B were assayed in triplicate for IL-2 production by ELISA.