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. 2000 Aug 29;97(18):10150–10155. doi: 10.1073/pnas.97.18.10150

Figure 4.

Figure 4

Expression of the 5C.C7 variable domains Vα11 and Vβ3 in 5C.C7 TCR transgenic mice on the B10.BR background or crossed with 129 mice. (A) Reduced 5C.C7 TCR expression in naive lymph node cells of 5C.C7 TCR transgenic mice crossed with 129 vav−/− mice (Upper). After these cells were primed with irradiated B10.BR splenocytes and 3 μM MCC peptide for 5–7 days, only cells positive for both Vα11 and Vβ3 survived (Lower). Naive and primed cells were stained for Vα11 and Vβ3 on different days. Minor differences in staining efficiency may therefore account for the differences in the absolute Vα11 staining intensity between naive and primed cells. (B) Comparable 5C.C7 TCR expression in lymph node cells of 5C.C7 TCR transgenic mice crossed with 129 wild-type or 129 vav−/− mice. Irrespective of the vav genotype, normal levels of the 5C.C7 TCR have been reduced to 6–11% by negative selection, presumably by a superantigen from the 129 background (Upper). After these cells were primed as described in A, only Vα11 and Vβ3-positive cells survived (Lower).