Figure 6.

MHC II accumulation during superantigen-mediated T cell stimulation differs significantly between vav^+/+ and vav^−/− cells and to a lesser extent between vav^+/+ and vav^+/− cells. I-E^k-GFP transfected A20 B cell lymphoma cells were loaded with 10 μM SEA or SEE and were used to stimulate either vav^+/+, vav^+/−, or vav^−/− T cells. The I-E^k–GFP accumulation at the T cell/APC interface was analyzed as described in Fig. 2. The number of T cell/APC couples analyzed in each experiment for vav^+/+, vav^+/−, and vav^−/− cells is n = 33, n = 30, and n = 35, respectively. The data are derived from three independent sets of experiments.