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GABA stimulated HCC cell proliferation through GABRA3. HepG2/Si-1 and HepG2/Si-Mock cells were treated with or without GABA (20 μmol/L). A: MTT assay of HepG2/Si-Mock cells treated with or without GABA. (n = 6, P < 0.05); B: MTT assay of HepG2/Si-1 cells treated with or without GABA. (n = 6); C and D: Soft agar colony formation assay. (n = 6, P < 0.05 for HepG2/Si-Mock vs HepG2/Si-Mock + GABA); E: Cell cycle measured by flow cytometry; F: Tumor volume in nude mice (mm3).
