Fig. 1.

Costitutive expression of CCL5 mRNA in SHR VSMCs is lower than the expression in WKY VSMCs, and Ang II inhibits CCL5 mRNA expression and protein production in SHR VSMCs. (A) After total RNAs were isolated from SHR or WKY VSMCs, real-time PCR was performed. Bars represent means±SD from three independent experiments. ^*p<0.05 vs. WKY VSMCs. (B, C) SHR VSMCs were untreated or treated with Ang II (0.1 µmol/l). After total RNAs and cell supernatants were isolated, real time PCR (B) and ELISA (C) were performed. Bars represent means±SD from three independent experiments. ^*p<0.05 vs. untreated VSMCs.