Fig. 4.

CCL5 increases the mRNA expression and production of AT₂ receptor in SHR VSMCs. SHR VSMCs were untreated (NT) or treated with CCL5 (100 ng/ml) and or Ang II (0.1 µmol/l) for 2 h. Then, total RNAs for real time PCR (A) and cell lysates for protein detection (B) were isolated. Cell lysates were separated on 10% SDS-polyacrylamide gels and then immunoblotted with AT₂ receptor antibody. Bars represent means±SD from four independent experiments ^*p<0.05 vs. untreated SHR VSMCs. The data shown are representative of three independent experiments.