Figure 3.

Virtual amino acid screening of the WETWV peptide. All 20 amino-acid types were introduced in the WETWV reference peptide at all five positions. For positions P-0, P-1, and P-4, the phage-display derived optimal residues (black) were identified among the most favorable based on the WaterMap energies. For P-2 and P-3, many different amino-acid types were comparable to or better than native residues, suggesting that energetic factors other than water displacement are important for affinity at these positions.