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Steady-State Kinetic Constants for Wild-Type and Mutant hTS
| Enzyme | kcat (s-1) | Km (μM) | kcat/Km (s−1 μM−1) |
|---|---|---|---|
| wt-hTS | 3.11 ± 0.04 | 2.59 ± 0.21 | 1.20 |
| M190K | 0.030 ± 0.01 | 179 ± 9 | 1.6 × 10−4 |
| M190E | 0.53 ± 0.25 | 0.96 ± 0.15 | 0.55 |
| A191K | 0.52 ± 0.06 | 0.85 ± 0.12 | 0.61 |
| L198P | 1.64 ± 0.09 | 15.63 ± 1.13 | 0.10 |
Kinetic constants were determined as described in “Methods.” Data are the means of at least three independent determinations.
