Figure 6.

Comparison of catalytic/binding residues among nylon oligomer hydrolase, EstB carboxylesterase, DD-peptidase, and Class C β-lactamase. (a) Superimposition of Hyb-24Y (green) and EstB carboxylesterase (PDB ID code: 1CI8) (pink). The main chain folding (ribbon diagram), side-chain (stick diagram) of catalytic/binding residues are shown. (b) Superimposition of the Hyb-S4M94-A¹¹²/Ald complex (orange) with the R61 DD-peptidase/glycyl-l-α-amino-ɛ-pimelyl-d-alanyl-d-alanine (designated as GAPAA) complex (PDB ID code: 1IKG) (pink). (c) Superimposition of the Hyb-S4M94-A¹¹²/Ald complex (orange) with wild-type Class C β-lactamase/m-nitrophenyl-2-(2-aminothiazol-4-yl)-2-[(Z)-methoxyimino]acetylaminomethylphosphonate) (designated as PTX) complex (PDB ID code: 1RGY) (light green) and extended spectrum Class C β-lactamase/PTX complex (PDB ID code: 1RGZ) (navy).