Figure 3.

Deviation of the ¹³C chemical shifts of HP21 and HP36 from random coil values. Data are plotted as observed—random coil. (A) HP21 ¹³C^α, (B) HP36 ¹³C^α, (C) HP21 ¹³CO, (D) HP36 ¹³CO, (E) HP21 ¹³C^β, and (F) HP36 ¹³C^β. Data were collected at 12°C and pH 5.4 for HP21, and 25°C and pH 5.0 for HP36. Both samples contain 10 mM sodium acetate and 150 mM sodium chloride. Random coil values of Wishart et al. were used.⁴⁵