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. 2009 Nov 24;4(11):e7997. doi: 10.1371/journal.pone.0007997

Figure 2. Analysis of cap- and poly(A)-dependent translation of luc mRNAs in HIV-1 PR treated HeLa extracts.

Figure 2

HeLa extracts were treated with 20 ng HIV-1 PR. After 30 min, SQ 2.5 µM was added to the lysate. Simultaneously, extracts were programmed with 20 ng (+/G−), (+/G/+), (A/G/−), (A/G/+), (−/G/−) or (−/G/+) luc mRNAs, which are schematized in panel (A). As a control, a replicate was incubated with 2.5 µM SQ from the beginning of the reaction (grey bars). B, C and D) Luc activity was analyzed 30 min later and the data obtained from translation of each mRNA were plotted. E) Relative Luc activity obtained from each mRNA in presence and absence of HIV-1 PR was represented. S.D. were obtained from three independent experiments. *P<0.05; **P<0.01.