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. 2009 Nov 24;4(11):e7997. doi: 10.1371/journal.pone.0007997

Figure 3. Analysis of poly(A)-dependent translation of luc mRNAs in HIV-1 PR treated HeLa extracts.

Figure 3

HeLa (A), Krebs-2 (B) extracts or RRL (C) were treated with 20 ng HIV-1 PR for 30 min. Next, SQ 2.5 µM was added to the translation reaction to inhibit the protease activity. At this time point, extracts were programmed with 200 ng (−/−) or (−/+) mRNAs. As a control, a replicate reaction was incubated with 2.5 µM SQ from the beginning of incubation (grey bars). Luc activity was analyzed 30 min later and relative Luc activity from two independent experiments was represented. D) In parallel, RNAs were isolated from HeLa extracts and the amount of luc mRNA was determined by real-time RT-PCR. Relative luc mRNA levels were then represented. *P<0.05; **P<0.01.