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. 2009 Nov 24;4(11):e7997. doi: 10.1371/journal.pone.0007997

Figure 4. Restoration of poly(A)-dependent translation by addition of recombinant GST-PABP1 in HeLa extracts treated with HIV-1 PR.

Figure 4

HeLa lysates were incubated with or without 10 ng HIV-1 PR. The integrity of eIF4GI and PABP was determined by western blotting (A). After 30 min, 20 ng (−/G/+) (B), (+/−) (C), (+/+) (D), (−/+) (E) or (−/−) (F) luc mRNAs and 2.5 µM SQ was added to the extract. In a control reaction, SQ was present throughout the time course (bar 2). The extracts indicated were then supplemented with 25 ng GST-PABP1 or GST. Luc activity was measured 1 h later. The relative Luc activity obtained from (−/G/+) (B), (+/−) (C), (+/+) (D), (−/+) (E) or (−/−) (F) luc mRNAs was represented. *P<0.05; **P<0.01; ns, non-significant; C.p., putative cleavage product.