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Plot showing the percentage of cells above the isotype threshold from plateCore (x-axis) and FlowJo (y-axis) for donor 1 (red) and 2 (blue) in channels FL1-H through FL4-H. plateCore gating for Phycoerythrin (PE) conjugated antibodies (FL2-H) was consistently lower than FlowJo, resulting in more cells above the isotype gate.
