Fig. 2.

A functional Rev or IN is not required for the formation of Rev–IN complex. Co-IP experiments were performed using the appropriate specific antibodies from lysate obtained cells infected by: (A) IN D64N D116N HIV-1, (B) Rev M10 HIV-1 or (C) ΔRev HIV-1 on Rev10⁺ cells, (D) ΔRev HIV-1. The presence of Rev, IN and actin was detected as described in (A). All other experimental details as described in the Material and methods section.