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Elution profile of Smad1 MH1 (blue) run on a Superdex 75 gel-filtration column calibrated with molecular-weight standards (grey) and purified protein run on an SDS–PAGE gel. (a) Smad1 MH1 elutes as a single symmetric peak consistent with a molecular weight of ∼16 kDa. (b) 12% SDS–PAGE analysis showing molecular-weight markers (lane 1; kDa) and a pure Smad1 MH1 protein band (lane 2).
