Table 1. Crystal, data-collection and refinement statistics for FGF-1 crystal-packing mutants.
Values in parentheses are for the highest resolution shell.
| Wild type† | E81A/K101A | E81A/E82N/K101A | E81S/K101A | E81S/E82N/K101A | |
|---|---|---|---|---|---|
| Space group | C2221 | C2221 | C2221 | C2221 | C2221 |
| Unit-cell parameters (Å) | |||||
| a | 74.1 | 73.3 | 74.7 | 77.9 | 77.9 |
| b | 96.8 | 97.9 | 95.8 | 92.4 | 93.2 |
| c | 109.0 | 108.4 | 108.2 | 107.5 | 107.1 |
| Maximum resolution (Å) | 1.65 | 1.80 (1.91–1.80) | 1.85 (1.89–1.85) | 2.15 (2.20–2.15) | 2.10 (2.15–2.10) |
| Mosaicity (°) | 0.47 | 0.41 | 1.05 | 0.84 | |
| Molecules per ASU | 2 | 2 | 2 | 2 | 2 |
| Matthews coefficient (Å3 Da−1) | 2.96 | 2.92 | 2.91 | 2.91 | 2.92 |
| Total reflections | 274769 | 204098 | 89819 | 121150 | |
| Unique reflections | 34332 | 30785 | 21461 | 22850 | |
| Redundancy | 3.5 | 4.0 | 4.2 | 2.6 | |
| Completeness (%) | 93.9 (58.4) | 91.7 (54.8) | 99.8 (99.9) | 98.9 (88.4) | |
| I/σ(I) | 12.6 (2.9) | 16.2 (5.7) | 6.8 (3.1) | 12.5 (2.9) | |
| Rmerge (%) | 7.6 (34.6) | 5.3 (19.8) | 8.3 (38.0) | 6.4 (25.2) | |
| Rcryst (%) | 19.0 | 19.0 | 18.1 | 21.0 | |
| Rfree (%) | 21.5 | 22.6 | 22.5 | 24.9 | |
| R.m.s.d. bond length (Å) | 0.014 | 0.016 | 0.010 | 0.011 | |
| R.m.s.d. bond angle (°) | 1.67 | 1.73 | 1.46 | 1.50 | |
| Ramachandran plot‡ | |||||
| Favored (%) | 97.5 | 96.8 | 97.1 | 96.8 | |
| Allowed (%) | 2.5 | 3.2 | 2.9 | 3.2 | |
| Outliers (%) | 0.0 | 0.0 | 0.0 | 0.0 | |
| PDB code | 3cqa | 3crg | 3crh | 3cri |