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. 2009 Oct 30;65(Pt 11):1145–1148. doi: 10.1107/S1744309109038238

Table 1. Purification of CPR-C2 from 1 g of wet E. coli cell pellet.

Step Total protein (mg) Total activity (U) Specific activity (U mg−1) Yield (%) Purification (fold)
Cell-free extract 149 1457 9.81 100 1
Ni Sepharose 6 FF 39.9 908 22.8 62.3 2.32
Superdex 75 HR 10/30 15.4 562 36.5 38.6 3.72