Figure 2.

Binding of AceK and ICDH was demonstrated by size-exclusion chromatography. The peaks corresponding to AceK alone (two peaks), ICDH alone and the AceK–ICDH mixture are labelled. There were two AceK peaks: AceK₁ is aggregate and AceK₂ is a dimer. The eluate of the AceK–ICDH peak was analyzed by SDS–PAGE. Lanes on the gel are vertically matched to their eluting fractions. The peak fractions 4, 5 and 6 were pooled and concentrated for crystallization.