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SDS–PAGE analysis of the fructofuranosidase from Sw. occidentalis. Lane 1, culture filtrate expressing fructofuranosidase activity before DEAE-Sephacel column chromatography pH 7; lane 2, after DEAE-Sephacel column chromatography. Lane 3, S. cerevisiae cellular extract expressing SoInv; lane 4, the purified enzyme from this organism. The positions of molecular-mass markers are indicated (in kDa) on the left.
