Table 1. Primers used in cloning and mutagenesis.

No.	Mutations	Direction	Sequence	Restriction site
1	Wt FL†	Forward	TGAGGATCCATGGCTTCTGGAGCCGATTC	BamHI
2	Wt FL†	Reverse	TCAAGATCTGCCATACAGGTCATCATCATTGTC	BglII
3	Wt N-D1	Forward	ACCTGGGAAGACATCGGGGGCAGATCTCATCACCATCACCATC	BglII
4	Wt N-D1	Reverse	GATGGTGATGGTGATGAGATCTGCCCCCGATGTCTTCCCAGGT	BglII
5	R53A	Forward	GAATTGCAGTTGTTCCGCGGTGACACAGTGTTGC	—
6	R53A	Reverse	GCAACACTGTGTCACCGCGGAACAACTGCAATTC	—
7	R86A	Forward	CTTGTTCTGATGAGAAGATTCGGATGAATGCTGTTGTTCGGAATAACCTTCG	—
8	R86A	Reverse	CGAAGGTTATTCCGAACAACAGCATTCATCCGAATCTTCTCATCAGAACAAG	—
9	R95G	Forward	GTTCGGAATAACCTTCGTGTAGGTCTAGGGGATGTCATCAGCATCC	—
10	R95G	Reverse	GGATGCTGATGACATCCCCTAGACCTACACGAAGGTTATTCCGAAC	—
11	R155H	Forward	GGAGACATTTTTCTTGTCCATGGTGGGATGCGTGCTGTG	—
12	R155H	Reverse	CACAGCACGCATCCCACCATGGACAAGAAAAATGTCTCC	—

^†Wild-type full-length p97.