| Temperature (K) |
293 |
293 |
293 |
293 |
| Crystal-growth time |
1 week |
2 d |
2 weeks |
2–4 d |
| Crystallization solution |
0.1 M sodium cacodylate pH 6.5, 0.01 M magnesium acetate, 1.3 M lithium sulfate |
0.1 M bicine pH 9, 0.15 M sodium chloride, 4%(w/v) PEG 550 MME |
0.1 M Na HEPES pH 7.5, 0.2 M calcium chloride, 22%(w/v) PEG 400 |
0.1 M Na HEPES pH 7.5, 4%(w/v) PEG 4000, 30%(v/v) MPD |
| Crystal dimensions (µm) |
60 × 60 × 60 |
300 × 30 × 30 |
150 × 100 × 100 |
200 × 200 × 20 |
| Space group |
I23 |
P61212 |
C2221
|
C2 |
| Unit-cell parameters (Å, °) |
a = b = c = 327.2, α = β = γ = 90 |
a = b = 196.2, c = 281.8, α = β = 90, γ = 120 |
a = 89.5, b = 187.4, c = 149.0, α = β = γ = 90 |
a = 163.9, b = 109.6, c = 102.0, α = γ = 90, β = 120.4 |
| Unit-cell volume (Å3) |
35 × 106
|
9 × 106
|
2.5 × 106
|
1.6 × 106
|
| No. of asymmetric units (ASUs) in cell |
24 |
12 |
8 |
2 |
| Range of possible No. of molecules in ASU |
5–14 |
3–8 |
1–3 |
1–4 |
| No. of molecules in ASU |
6 |
3 |
2 |
2 |
|
VM (Å3 Da−1) |
4.58 |
4.88 |
2.72 |
3.44 |
| Solvent content (%) |
73.15 |
74.81 |
54.76 |
64.26 |
| No. of biological units in ASU |
3 |
1.5 |
1 |
1 |
| No. of crystals tested |
50 |
100 |
20 |
20 |
| Resolution limit (Å) |
4.6 |
4.0 |
3.4 |
2.7 |
