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SDS–PAGE analysis of the final purification stage of Stk1. Lanes 1 and 2, elution fractions of Stk1 after ion-exchange chromatography carried out with a MonoQ 5/50 Tricorn column. Lane 3, molecular-mass markers (kDa). Fractions were analysed on 10% SDS–PAGE and stained with Coomassie Blue.
