Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of overexpressed PepcA. Lane 1, whole cell extract before induction of PepcA expression; lane 2, whole cell extract after IPTG induction; lane 3, molecular-weight markers; lane 4, soluble portion of the extract; lane 5, insoluble portion of the extract; lane 6, purified PepcA; lane 7, molecular-weight markers with mass indicated in kDa. The calculated molecular weight of His10-tagged PepcA is 62.3 kDa.
