TABLE 2.

Modulation of Transcriptional Gene Expression by Phytochemicals in Cultured Human Primary Hepatocytes

Treatment	CYP1A1	CYP1A2	CYP3A4	CYP3A5	mEH	GSTM1a	GSTT1a
Control	1.0	1.0	1.0	1.0	1.0	1.0	1.0
DIM, 10μM	113 ± 16 (8)**	24.9 ± 3.1 (8)**	1.1 ± 0.2 (8)	1.1 ± 0.2 (8)	1.4 ± 0.3 (8)	0.79 ± 0.36 (4)	1.2 ± 0.4 (6)
DIM, 50μM	625 ± 156 (8)**	90.2 ± 20.1 (8)**	1.2 ± 0.2 (8)	1.1 ± 0.1 (8)	2.0 ± 0.9 (8)	0.56 ± 0.09 (4)*	2.0 ± 1.2 (6)
8PN, 10μM	1.6 ± 0.2 (8)	2.0 ± 0.3 (8)*	1.2 ± 0.3 (8)	0.8 ± 0.2 (8)	4.1 ± 1.6 (8)	1.4 ± 0.3 (4)	0.9 ± 0.2 (6)
8PN, 25μM	1.7 ± 0.4 (8)	2.3 ± 0.4 (8)*	0.8 ± 0.1 (8)	0.7 ± 0.2 (8)	2.2 ± 0.5 (8)	1.1 ± 0.2 (4)	0.9 ± 0.1 (6)

Note. Hepatocytes were treated with two doses of DIM or 8PN for 48 h, and mRNA levels of genes listed were determined by quantitative RT-PCR analysis as described in the “Materials and Methods” section. Control represents non-modulated transcription level following solvent control treatment for 48 h. Values are expressed as fold alteration relative to solvent control (with the control equal to 1) and represent means of fold alteration values and SEs from hepatocytes from N individual preparations (N is displayed in parentheses).

^aincludes only samples that had a positive genotype (e.g., were not homozygous null for the gene deletion).

*Denotes 0.01 < p < 0.05, **denotes 0.001 < p < 0.01, ***denotes p < 0.001.