FIG. 5.

Radiographs of the penis and micrographs of the body of the penis in adult rats treated neonatally with oil (control) or EE at a dose of 10 ng or 100 ng or 1 μg or 10 μg/rat on every other day from postnatal days 1 to 11. In radiographs, note grossly mal-developed penis in the 1-μg and 10-μg groups and dose-dependent underdevelopment of the proximal os penis (PO) and distal os penis (DO) in the > 100-ng groups as compared with controls and the 10-ng group. In micrographs, compared with the control and 10-ng groups, note increased number of fat cells (arrows) in the corpora cavernosa (CC) of the 100-ng group, and a nearly complete replacement of cavernous spaces and adjacent smooth muscle cells and collagen fibers by fat cells in the corpora cavernosa of the 1-μg and 10-μg groups. CS, corpus spongiosus; TA, tunica albuginea capsule; BV, blood vessel in the intercrural septum; N, nerve. En block staining with osmium tetroxide, followed by H&E staining of paraffin sections. All micrographs are of the same magnification.