FIG. 6.

Radiographs of the penis and micrographs of the body of the penis in adolescent and adult rats treated neonatally with EE or DES at a dose of 100 ng/rat every day from postnatal days 1 to 6. In radiographs, compared to controls, note that the proximal os penis (PO) is less developed and the distal os penis (DO) is not developed at adolescence in both DES and EE groups. Similarly, both groups had somewhat less developed DO at adulthood. In micrographs, note that both DES and EE treatments caused similar level of fat accumulation (arrows) in the copora cavernosa at adolescence and adulthood. CC, corpora cavernosa; CS, corpus spongiosus; TA, tunica albuginea capsule; BV, blood vessel; N, nerve. En block staining with osmium tetroxide, followed by H&E staining of paraffin sections. All micrographs are of the same magnification.