FIG. 2.

Differential exposure to Mn in juvenile and adult C57Bl/6J mice induces distinct patterns of microgliosis in the basal ganglia. Mice were exposed to 0, 10, or 30 mg/kg MnCl₂ by daily intragastric gavage as juveniles, adults, or both juveniles and adults. Multiple brain regions in the basal ganglia were assessed for activation of microglia by immunohistochemical staining for Iba-1 including the St, Gp, and SNpr. Representative images of the SNpr are depicted for control mice and mice exposed to 30 mg/kg MnCl₂ as (A) juveniles, (B) adults, and (C) both juveniles and adults. Arrowheads indicate cell bodies. Scale bar = 10 μm.