FIG. 3.
Activated astrocytes in C57Bl/6J mice express NOS2 following differential exposure to Mn as juveniles and adults. Astroglial expression of NOS2 was assessed via co-immunofluorescence in multiple regions of the basal ganglia from mice exposed to Mn. Representative images of the SNpr are presented from control mice and those treated with 30 mg/kg MnCl2 as (A) juveniles, (B) adults, and (C) both juveniles and adults. Images of GFAP and NOS2 expression are shown in green and red channels, respectively, and cell nuclei are highlighted by staining with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) in blue. Yellow areas in merged images indicate colocalization of NOS2 expression in GFAP-positive astrocytes in mice exposed to Mn. Scale bar = 10 μm.