FIG. 5.

Activated microglial cells in C57Bl/6J mice express NOS2 following differential exposure to Mn as juveniles and adults. Microglial expression of NOS2 was assessed via co-immunofluorescence in the St, Gp, and SNpr from mice exposed to Mn. Representative images of the SNpr are presented from control mice and those treated with 30 mg/kg MnCl₂ as (A) juveniles, (B) adults, and (C) both juveniles and adults. Images of Iba-1 and NOS2 expression are shown in green and red channels, respectively, and cell nuclei were highlighted by staining with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) in blue. Merged images indicate colocalization of NOS2 expression in Iba-1–positive microglia in mice exposed to Mn. Scale bar = 10 μm.