FIG. 7.

Mn exposure increases levels of 3-NTyr protein adducts in basal ganglia neurons. To detect modification of neuronal proteins by peroxynitrite (ONOO⁻) derived from increased production of NO by activated glia, serial sections from the SNpr of (A) juvenile, (B) adult, and (C) both juvenile and adult mice exposed to 0 and 30 mg/kg MnCl₂ were stained with antibodies against the general neuronal marker MAP-2 (green) and 3-NTyr (red) and were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to identify cell nuclei (blue). Representative images of 3-NTyr–modified proteins indicate colocalization of 3-NTyr adducts with both neuronal soma and dendrites in the SNpr of Mn-treated juvenile mice. Scale bar = 10 μm.