FIG. 8.

Quantitative analysis of 3-NTyr adducts indicates that modification of neuronal proteins by peroxynitrite correlates with regional patterns of glial activation and NOS2 expression. Specific brain regions were evaluated for colocalization of MAP-2 and 3-NTyr by immunofluorescence in control mice and those exposed to 30 mg/kg MnCl₂. Graphs indicate quantification of levels of 3-NTyr protein adducts in neurons from St, Gp, and SNpr of mice exposed as (A) juveniles, (B) adults, and (C) juveniles and adults. Three serial sections were stained for each brain region per hemisphere from an average of three mice per treatment group. For quantitative cell counts, three microscopic fields were evaluated per brain region from each serial section, totaling n = 9 per treatment group. Data indicate increased protein nitration in the neurons of the Gp and SNpr. Different letters denote significant differences between treatments, p < 0.05.