FIG. 3.

Role of complexes I and III in redox cycling herbicide-induced mitochondrial H₂O₂ production. Isolated rat brain mitochondria were supplemented with malate + glutamate in the presence of (A) PQ, (B) DQ, or (C) BV. Inhibitors of the respiratory chain, rotenone (5 μg/ml; white bars) or antimycin A (10μM; gray bars), were added as indicated. Data expressed as H₂O₂ production rates in nanomolar per minute + SEM, n = 3–4. Bars with different letters indicate statistical significance from one another (p < 0.05, one-way ANOVA with Tukey’s posttest).