FIG. 5.
CD40L-induced activation of mouse B cells. Mouse B cells (1.5 × 106 cell/culture) were cocultured with irradiated CD40L-L cells (5 × 104 cell/culture) in the presence of recombinant mouse IL-2, IL-6, and IL-10 for up to 5 days, and CD40L stimulation was removed on day 3. Cells were harvested at the indicated time points and assessed by flow cytometry. Dead cells were identified by staining with Live/Dead near-infrared Staining Kit and excluded from data analysis. These data are representative of two separate experiments with three replicates per time point.