FIG. 6.

CD40L-induced activation of human B cells. Human CD19⁺CD27⁻ naive B cells (1.5 × 10⁵ cell/culture) were cocultured with irradiated CD40L-L cells (1.5 × 10³ cell/culture) in the presence of recombinant human IL-2, IL-6, and IL-10 for up to 6 days, and CD40L stimulation was removed on day 4. Cells were harvested at indicated time points and assessed by flow cytometry. Dead cells were identified by staining with Live/Dead near-infrared Staining Kit and excluded from data analysis. These data are representative of two separate experiments (each experiment used B cells from one individual donor) with three replicates per time point.