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Fission of precurved membrane tubes mediated by Dyn1 I533A. Time-lapse fluorescence images showing the effect of addition of either 0.5 μM Dyn1 WT or Dyn1 I533A to membrane tethers (typically ∼50–100-nm diameter) pulled out of RhPE-labeled SUPER templates in the presence of GTP (1 mM final). See Supplemental Movie 2 for entire sequence.
