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. 2009 Nov 15;20(22):4640–4651. doi: 10.1091/mbc.E09-05-0429

Figure 2.

Figure 2.

SGIP1-α is a Syp1p homolog. (A) Sequence alignment of Syp1p, SGIP1-α (Mus musculus, NP_659155.1), FCHo1 (H. sapiens, NP_055937.1), and FCHo2 (H. sapiens, NP_620137). Key to alignment: red, complete consensus; blue, consensus of two or three sequences; #, conserved as D/E.; and !, conserved as I/V. (B) Predicted domain structures of Syp1p, SGIP1-α, FCHo1, and FCHo2. MP, membrane phospholipid-binding domain; SAFF, a PFAM predicted domain. (C) Colocalization of SGIP1-α-GFP and DsRed-clathrin (mouse clathrin light chain a) at the cell surface by TIRF microscopy in Swiss 3T3 cells. Scale bar, 2 μm. (D) Dynamic cell surface localization of SGIP1-α-GFP and DsRed-clathrin by TIRF microscopy. Three representative endocytic site assembly (top) and disassembly (bottom) events are shown. For each event, a montage of DsRed-clathrin and SGIP1-α-GFP recruitment to endocytic sites is shown (frame interval, 12 s), as well as quantification of fluorescence intensity over time (frame interval, 2 s). The region of the graph corresponding to the montage is outlined in gray.