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. 2009 Nov 15;20(22):4640–4651. doi: 10.1091/mbc.E09-05-0429

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© 2009 by The American Society for Cell Biology

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Figure 3. — The endocytic role of Syp1p and Ede1p. (A) Patch lifetimes of Las17-GFP, Sla1-GFP, Sla2-GFP, Ent1-GFP, and Ent2-GFP in wild-type, syp1Δ, ede1Δ, and syp1Δ ede1Δ cells (strains DDY2736, -3696, -3697, -3700, -3701, -3798, and -3872–3885). n = 30 patches for each strain. Error bars, SD. Frame rate was 1 frame per second. (B) Kymographs of representative Las17-GFP, Sla1-GFP, Sla2-GFP, Ent1-GFP, and Ent2-GFP patches from epifluorescence movies of wild-type, syp1Δ, ede1Δ, and syp1Δ ede1Δ cells (the same strains listed in A). Patches are oriented so the outside of the cell is on top. (C) Epifluorescence images of live wild-type syp1Δ, ede1Δ, and syp1Δ ede1Δ cells expressing Ent1-GFP or Ent2-GFP (strains DDY3696, -3697, and -3880–3885). Top, small-budded cells; bottom, large-budded cells. Scale bars, 2 μm.