Figure 5.

Members of the Crb3 complex, but not Par3–aPKC complex, are recruited to Crb3a-positive endosomes. MDCKII cells were embedded in Geltrex as previously described, fixed at different times, and stained with the indicated antibodies. Single Z-sections are displayed. (A and B) Exogenously expressed GFP-PALS1 and GFP-PATJ colocalized with Crb3a. (C and D) Par3 and aPKCζ do not initially colocalize with GFP-Crb3a, but get enriched at the newly forming TJ and/or apical membrane between the two daughter cells and at the emerging lumen.