Table 1.
CLIC4 translocation, Cl−-mediated membrane depolarization, and RhoA-mediated cytoskeletal contraction under various conditionsa
| Agonist/treatment | CLIC4 translocation | Cl− channel activation (depolarization) | Cytoskeletal contraction (RhoAmediated) |
|---|---|---|---|
| Serum (5%) | ++ | ++ | ++ |
| LPA, S1P, TRPb | ++ | ++ | ++ |
| Bradykinin,cisoprotenerol | − | − | − |
| LPA | |||
| + Pertussis toxin(200 ng/ml) | ++ | ++ | ++ |
| + Gα13 or RhoAknockdown | − | − | − |
| + C3 transferase(30 μg/ml) | − | − | − |
| + Y-27632 (100 μM) | ++ | ++ | − |
| + Latrunculin A(1 μM) | − | ++ | − |
| + CLIC4knockdown | ++ | ++ |
a++, positive response; −, no response.
bLigand concentrations: LPA, S1P, bradykinin, isoprotenerol, 1 μM; TRP, 50 μM.
cSee Supplemental Figure S1.